Introduction to the OptiPrep Methodology

Application Sheets

There are four indexes described below: 

Cell-index - 51 Application Sheets; comprising 4 on gradient management and 47 on the purification of different types of mammalian and non-mammalian cells.

 

Macromol-index - 13 Application Sheets; comprising 5 on gradient management; and 8 on proteins, RNA, DNA and polysaccharides, protein complexes and protein-nucleic acid complexes, proteoliposomes and plasma lipoproteins.

 

Submem-index - 67 Application Sheets; comprising 9 on gradient management and ancillary techniques (e.g. homogenization methods). There are 58 on the purification different types of mammalian and non-mammalian subcellular membrane compartments and organelles.

 

Virus-index - 38 Application Sheets; comprising 5 on gradient management; one on purification strategies and 32 on purification of animal and plant cell viruses and bacteriophages. These Application Sheet indexes are presented on pages 2-14 of this document.

 

Reference Lists

There are four indexes described below:

 

Cell-index - this comprises 9 types of mammalian cells.

 

Macromol-index - 2 files include proteins, RNA, DNA and polysaccharides, protein complexes and protein-nucleic acid complexes, proteoliposomes and plasma lipoproteins.

 

Submem-index - this comprises 15 files and includes all major organelles and subcellular membrane compartments from mammalian cells, plants, algae, fungi, protozoa and also membrane vesicles from bacteria.

 

Virus-index - this comprises 8 files and includes 62 viruses listed according to the Baltimore Classification system. Plant cell viruses and bacteriophages are also included. Because of the huge number of rAAV papers Group II is split into two sections RV02-1 and RV02-2. These Reference List indexes are presented on pages 15 and 16 of this document