OPTIPREP APPLICATION SHEET INDEX VIRUSES
Since the first papers reporting the use of density gradients, prepared from OptiPrep were
published in 1998-1999, Application Sheets providing detailed protocols and reviews of
methodologies have been produced and updated, on an ad hoc basis, as new information became
available in scientific journals. Today OptiPrep has been used for the purification and analysis
of over 80 types of virus; we have therefore now reorganized all of these Application Sheets.
All of the general information on gradient production and analysis plus pre-gradient virus
concentration are contained in Application Sheets V01-V06.
All the virus gradient protocols have been rearranged into the seven groups of the Baltimore
Virus Classification scheme (V07-V36).
Within each group, Families (sometimes preceded by the Order name), and where appropriate
Subfamilies, are listed alphabetically in italics. Within each Family or Subfamily individual
viruses are also listed alphabetically.
Click on the relevant [APPLICATION SHEET] for a detailed protocol of virus isolation.
In some cases more than one Application Sheet for a specific virus type may be provided, if
significantly different practical strategies are available.
Protocols for one particular virus in a Group may be applicable to other viruses in the same group
with a similar overall structure and composition, but this can only be determined experimentally.
Yeast retrotransposons, although not part of Baltimore Virus Classification system, are
considered similar in structure to retroviruses. They are included in Group VI listings.
Plant Virus and Bacteriophage Application Sheets can accessed from this index after the entry
for Group VII viruses.
1. Gradient preparation and analysis and virus concentration
Preparation of density gradient solutions [APPLICATION SHEET V01]
Preparation of discontinuous and continuous gradients [APPLICATION SHEET V02]
Preparation of self-generated gradients [APPLICATION SHEET V03]
Harvesting gradients [APPLICATION SHEET V04]
Analysis of gradients [APPLICATION SHEET V05]
Concentration of virus samples [APPLICATION SHEET V06]
2. Virus purification
Group I (ds)DNA viruses
Adenoviridae
Adenovirus [APPLICATION SHEET V07]
Helper-dependent virus, separation from [APPLICATION SHEET V07]
Asfaviridae
African swine fever virus [APPLICATION SHEET V09]
Baculoviridae
Baculovirus vectors [APPLICATION SHEET V08]
Herpesviridae
Cytomegalovirus
Self-generated gradient [APPLICATION SHEET V08]
Pre-formed gradient [APPLICATION SHEET V09]
Epstein-Barr virus
Self-generated gradient [APPLICATION SHEET V08]
Pre-formed gradient [APPLICATION SHEET V09]
Herpes simplex virus
Self-generated gradient [APPLICATION SHEET V08]
Pre-formed gradient [APPLICATION SHEET V09]
Herpes virus vectors [APPLICATION SHEET V08]
Rhadinovirus [APPLICATION SHEET V09]
Iridoviridae
Iridovirus [APPLICATION SHEET V13]
Papillomaviridae
Human papillomavirus [APPLICATION SHEET V10]
Phycodnaviridae
Chlorovirus [APPLICATION SHEET V13]
Polyomaviridae
BK virus [APPLICATION SHEET V11]
JC virus [APPLICATION SHEET V11]
Merkel cell carcinoma virus [APPLICATION SHEET V11]
Simian virus 40 (SV40) [APPLICATION SHEET V11]
Poxviridae
Molluscipoxvirus [APPLICATION SHEET V12]
Vaccinia virus [APPLICATION SHEET V12]
Group II (ss)DNA viruses
Anelloviridae
Torque teno virus [APPLICATION SHEET V15]
Parvoviridae
Densovirinae
Dependovirus
Avian adeno-associated virus [APPLICATION SHEET V14]
Recombinant adeno-associated virus [APPLICATION SHEET V14]
Parvovirinae
Minute virus of mice [APPLICATION SHEET V15]
Parvovirus [APPLICATION SHEET V16]
Group III (ds)RNA viruses
Reoviridae
Birnavirus
Espirito Santo virus [APPLICATION SHEET V17]
Sedoreovirinae
Rotavirus [APPLICATION SHEET V17]
Seadornavirus [APPLICATION SHEET V17]
Spinareovirinae
Dinovernavirus [APPLICATION SHEET V17]
Group IV ((+)ss)RNA viruses
Caliciviridae
Lagovirus
Rabbit haemorrhagic fever virus [APPLICATION SHEET V18]
Norovirus
Norwalk virus [APPLICATION SHEET V18]
Group IV ((+)ss) RNA viruses (contd.)
Flaviviridae
Flavivirus
Dengue virus [APPLICATION SHEET V19]
West Nile virus [APPLICATION SHEET V19]
Yellow fever virus [APPLICATION SHEET V19]
Hepacivirus
Hepatitis C virus
Pre-formed gradient [APPLICATION SHEET V19]
Self-generated [APPLICATION SHEET V20]
Hepevirus
Hepatitis E virus [APPLICATION SHEET V19]
Pestivirus
Bovine diarrhea virus [APPLICATION SHEET V19]
Nidovirales - Arteriviridae
Porcine reproductive & respiratory syndrome virus [APPLICATION SHEET V21]
Nidovirales - Coronaviridae
SARS coronavirus [APPLICATION SHEET V21]
Togaviridae
Alphavirus
Chikungunya virus [APPLICATION SHEET V22]
Semliki Forest virus [APPLICATION SHEET V22]
Sindbis virus [APPLICATION SHEET V22]
Venezuelan equine encephalitis virus [APPLICATION SHEET V22]
Rubivirus
Rubella virus [APPLICATION SHEET V22]
Tombusviridae
Dianthovirus
Red clover mosaic virus [APPLICATION SHEET V22]
Group V ((-)ss) RNA viruses
Arenaviridae
Arenavirus
Lassa virus [APPLICATION SHEET V23]
Tacaribe virus [APPLICATION SHEET V23]
Bunyaviridae
Hantavirus
Hantavirus [APPLICATION SHEET V24]
Orthobunyavirus
Bunyamweravirus [APPLICATION SHEET V24]
Phlebovirus
Rift valley fever virus [APPLICATION SHEET V24]
Filoviridae
Ebola virus [APPLICATION SHEET V25]
Orthomyxoviridae
Influenza virus [APPLICATION SHEET V26]
Rhabdoviridae
Lyssavirus
Rabies virus [APPLICATION SHEET V27]
Vesiculovirus
Vesicular stomatitis virus [APPLICATION SHEET V27]
Paramyxoviridae
Paramyxovirinae
Avulavirus
Newcastle disease virus [APPLICATION SHEET V28]
Morbillivirus
Measles virus [APPLICATION SHEET V28]
Respirovirus
Swine paramyxovirus [APPLICATION SHEET V28]
Pneumovirinae
Human respiratory syncytial virus [APPLICATION SHEET V28]
Group VI (ss)RNA-RT viruses
Retroviridae
Alpharetrovirus
Rous sarcoma virus [APPLICATION SHEET V29]
Betaretrovirus
Mason-Pfizer monkey virus (Simian retrovirus) [APPLICATION SHEET V30]
Deltaretrovirus
Human T cell lymphotropic virus [APPLICATION SHEET V31]
Gammaretrovirus
Moloney murine leukaemia virus [APPLICATION SHEET V33]
Murine oncornavirus [APPLICATION SHEET V32]
Lentivirus
Feline immunodeficiency virus [APPLICATION SHEET V34]
Human immunodeficiency virus [APPLICATION SHEET V34]
Spumaretroviridae
Spumavirus
Foamy virus [APPLICATION SHEET V35]
Yeast retrotransposons [APPLICATION SHEET V37]
Group VII (ds)RNA-RT
Hepadnaviridae
Orthohepadnavirus
Hepatitis B [APPLICATION SHEET V36]
Plant viruses [APPLICATION SHEET V37]
Bacteriophages [APPLICATION SHEET V38]
Latest news
Axis-Shield will exhibit at the ASCB meeting in San Francisco December 3-7. You will find us in booth 418
We have recently posted a new package insert for Polymorphprep (see Products in the main menu). In addition to a new figure, we have added a new paragraph; Important practical and sample notes.
We have decided to discontinue the product NycoPrep 1.077, 4x250 ml. We still have lot no. 12GLS12 in stock, with an extended expiry date of November 2017.